Studying chemical mixtures in real-life samples is challenging due to the difficulty of linking specific substances to particular effects within the mixture. HT-EDA can be used to focus on the adverse effects produced by the entire mixture and then trace back to identify the chemicals responsible. The implementation of our in-house developed faction collector (Fractiomate™) has allowed the field of EDA to use high-throughput workflows to pinpoint specific substances responsible for observed biological effects.

Our lab is pioneering the use of HT-EDA to study complex mixtures contributing in projects such as Panoramix, and TKIdentification. In these projects real-life complex samples are extracted and micro-fractionated for their simultaneous use in bioassays and chemical analysis. In addition, our research group is actively involved in the activities proposed by the NORMAN society (Network of reference laboratories, research centres and related organisations for monitoring of emerging environmental substances), participating in specific cross-working group activities such as non-target screening (NTS), and co-coordinating the WG3: EDA.

Projects:

Panoramix (Marja Lamoree, Maria Margalef, Timo Hamers) “Providing risk assessments of complex real-life mixtures for the protection of Europe’s citizens and the environment”

TKIdentification (Marja Lamoree, Timo Hamers) “Platform for combined high-throughput Effect-Directed Analysis for the assessment of occurrence, fate and activity of Tyrosine Kinase Inhibitors as a novel class of pharmaceutical residues in the Dutch water cycle”

NORMAN (Frederic Béen, Marja Lamoree, Maria Margalef) “Working Group 3: Effect-directed analysis for hazardous pollutants identification”