These new tools allow real-time measurements of the release of the neurotransmitter histamine with sub-second kinetics and high specificity from brain slices and in intact brains of living mice.
Histamine is a key neurotransmitter involved in a wide range of physiological and pathological processes (e.g. regulation of sleep/wakefulness, learning, food intake) in both the central and peripheral nervous systems. Using these new fluorescent sensors, real-time histamine release in different brain areas was measured during the sleep-wake cycle in freely moving mice. The ability to directly observe histamine release in real time provides important new tools to study the role of histamine in complex brain circuits.
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