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Molecular Neurobiology

The course aims at providing knowledge of concepts of Molecular Neurobiology in the context of investigating and understanding gene function in the nervous system.

Course Objective

  • General introduction to molecular biological principles
  • Various quantification methods (e.g. quantitative PCR, Western blotting)
  • Screening techniques (hybridization, micro arrays, SAGE, deep sequencing)
  • Structure-function research (mutagenesis, 3D structure analysis)
  • Genetic intervention studies (viral vectors, transgenesis, RNAi, peptide-mimetics)
  • Protein-protein interaction (immunoprecipitation, yeast two hybrid)
  • Proteomics technology (protein identification and quantification, determining phosphorylation  with MS)
  • Cellomics technology (cellular high-content screening)
  • Genome projects and the use of electronic databases (Entrez-based)
  • Various examples from current research involving the above topics

Study Characteristics

  • Discipline: Neurosciences
  • Type of education: In class
  • Academic skill: Research
  • Graduate School: ONWAR: Graduate School Neurosciences Amsterdam Rotterdam (www.onwar.nl)
  • Start date: bi-annual course, next course (maybe) in 2020, October
  • End date: bi-annual course
  • Minimum number of students: 15
  • Maximum number of students: 40
  • Admission criteria: PhD-students ONWAR (see also website ONWAR). External (neuroscience) PhD-students: fee is EUR 500.  ONWAR-PhD-students always have priority.
  • Concluding assessment: No
  • With Certificate: Yes (for non-ONWAR-PhD-students)
  • Schedule info: 5 days
  • Number of lessons: N/A
  • Total course duration in hrs.: N/A
  • Sign up period: Continuously
  • Anticipated hrs of study: N/A
  • Available to: see admission criteria
  • Course Objective & Study Characteristics

    Course Objective

    • General introduction to molecular biological principles
    • Various quantification methods (e.g. quantitative PCR, Western blotting)
    • Screening techniques (hybridization, micro arrays, SAGE, deep sequencing)
    • Structure-function research (mutagenesis, 3D structure analysis)
    • Genetic intervention studies (viral vectors, transgenesis, RNAi, peptide-mimetics)
    • Protein-protein interaction (immunoprecipitation, yeast two hybrid)
    • Proteomics technology (protein identification and quantification, determining phosphorylation  with MS)
    • Cellomics technology (cellular high-content screening)
    • Genome projects and the use of electronic databases (Entrez-based)
    • Various examples from current research involving the above topics

    Study Characteristics

    • Discipline: Neurosciences
    • Type of education: In class
    • Academic skill: Research
    • Graduate School: ONWAR: Graduate School Neurosciences Amsterdam Rotterdam (www.onwar.nl)
    • Start date: bi-annual course, next course (maybe) in 2020, October
    • End date: bi-annual course
    • Minimum number of students: 15
    • Maximum number of students: 40
    • Admission criteria: PhD-students ONWAR (see also website ONWAR). External (neuroscience) PhD-students: fee is EUR 500.  ONWAR-PhD-students always have priority.
    • Concluding assessment: No
    • With Certificate: Yes (for non-ONWAR-PhD-students)
    • Schedule info: 5 days
    • Number of lessons: N/A
    • Total course duration in hrs.: N/A
    • Sign up period: Continuously
    • Anticipated hrs of study: N/A
    • Available to: see admission criteria

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