BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Vrije Universiteit Amsterdam//NONSGML v1.0//EN
NAME:PhD defense Y. Ma
METHOD:PUBLISH
BEGIN:VEVENT
DTSTART:20261117T094500
DTEND:20261117T111500
DTSTAMP:20261117T094500
UID:phd-defense-y-ma@8F96275E-9F55-4B3F-A143-836282E12573
CREATED:20261021T195153
LOCATION:Main building VU, 1105, Auditorium, De Boelelaan, 1081 HV, Amsterdam
SUMMARY:PhD defense Y. Ma
X-ALT-DESC;FMTTYPE=text/html: <html> <body> <p><p>Quantitative Label-F
 ree Higher Harmonic Generation Microscopy for 3D and 4D Analysis of T
 issue Structure and Dynamics</p></p> <p>"I used a label-free microsco
 py technique (HHGM) to visualize and analyze cells and the tissue mat
 rix around them (ECM) in models of the bone disease OI, the connectiv
 e tissue disease EDS and lung diseases. In this way, we unravel how t
 hese diseases work.</p><p>"HHGM delivers "direct pathology": cells an
 d structures can be seen in fresh tissue within minutes – much fast
 er than classic H&amp;E staining. Old methods such as immune staining
  or electron microscopy are static and time-consuming; Living cell st
 aining in turn brings harmful dyes and light damage, which can change
  cells and only allow short exposures.</p><p>"My goal: to further dev
 elop HHGM into dynamic four-dimensional (4D) images of living tissue,
  and to determine what is needed for this.</p><p>"We show that label-
 free HHGM is a good tool to investigate 3D cultures of connective tis
 sue cells (fibroblasts) and to measure collagen defects in the matrix
  that arise due to OI and EDS gene mutations.</p><p>"It also appears 
 that completely label-free 4DHHGM can record images of elastin and co
 llagen, contacts between cells and cell movement in fresh, precisely 
 cut lung sections (PCLS) from humans – recorded as videos over a pe
 riod of no less than five days."</p><p>More information on the thesis
  to follow.</p> </body> </html>
DESCRIPTION: Quantitative Label-Free Higher Harmonic Generation Micros
 copy for 3D and 4D Analysis of Tissue Structure and Dynamics "I used 
 a label-free microscopy technique (HHGM) to visualize and analyze cel
 ls and the tissue matrix around them (ECM) in models of the bone dise
 ase OI, the connective tissue disease EDS and lung diseases. In this 
 way, we unravel how these diseases work."HHGM delivers "direct pathol
 ogy": cells and structures can be seen in fresh tissue within minutes
  – much faster than classic H&amp;E staining. Old methods such as i
 mmune staining or electron microscopy are static and time-consuming; 
 Living cell staining in turn brings harmful dyes and light damage, wh
 ich can change cells and only allow short exposures."My goal: to furt
 her develop HHGM into dynamic four-dimensional (4D) images of living 
 tissue, and to determine what is needed for this."We show that label-
 free HHGM is a good tool to investigate 3D cultures of connective tis
 sue cells (fibroblasts) and to measure collagen defects in the matrix
  that arise due to OI and EDS gene mutations."It also appears that co
 mpletely label-free 4DHHGM can record images of elastin and collagen,
  contacts between cells and cell movement in fresh, precisely cut lun
 g sections (PCLS) from humans – recorded as videos over a period of
  no less than five days."More information on the thesis to follow.
END:VEVENT
END:VCALENDAR