Profiling elapid ven oms for coagulation and paralysis modulators by liquid chromatography with post-column in vitro/in vivo bioassays and mass spectrometry
Chemist Arif Arrahman has conducted research into the harmfu l effects of snake venom and how we can better understand and combat them. Snakebites remain a major health problem in many parts of the w orld and can lead to paralysis, blood clotting problems, and even dea th.
The research focused on the following questions: Which toxi ns in snake venom cause these effects, and can we quickly identify th em? Arrahman also investigated whether a small drug candidate called varespladib can block dangerous venom components, particularly those that affect blood clotting.
The researcher demonstrated that ce rtain cobra venoms contain toxins that impair blood clotting, and tha t most of these toxins originate from a group of enzymes called PLA� �. The research also showed that varespladib can reduce or block thes e harmful effects in many cobra venoms. Furthermore, combining labora tory cell tests with zebrafish tests helped identify toxins that caus e paralysis by affecting ion channels.
This knowledge can help doctors and healthcare providers treat snakebite victims more effecti vely, especially in rural areas. The results also support the develop ment of varespladib as a potential first-aid treatment before patient s reach the hospital. Besides snakebites, pharmaceutical researchers can benefit from this, as toxins with potent effects on the body can inspire new drugs.
More information on the thesis
< /body> DESCRIPTION: Profiling elapid venoms for coagulation and paralysis mod ulators by liquid chromatography with post-column in vitro/in vivo bi oassays and mass spectrometry Chemist Arif Arrahman has conducted res earch into the harmful effects of snake venom and how we can better u nderstand and combat them. Snakebites remain a major health problem i n many parts of the world and can lead to paralysis, blood clotting p roblems, and even death.The research focused on the following questio ns: Which toxins in snake venom cause these effects, and can we quick ly identify them? Arrahman also investigated whether a small drug can didate called varespladib can block dangerous venom components, parti cularly those that affect blood clotting.The researcher demonstrated that certain cobra venoms contain toxins that impair blood clotting, and that most of these toxins originate from a group of enzymes calle d PLA₂. The research also showed that varespladib can reduce or blo ck these harmful effects in many cobra venoms. Furthermore, combining laboratory cell tests with zebrafish tests helped identify toxins th at cause paralysis by affecting ion channels.This knowledge can help doctors and healthcare providers treat snakebite victims more effecti vely, especially in rural areas. The results also support the develop ment of varespladib as a potential first-aid treatment before patient s reach the hospital. Besides snakebites, pharmaceutical researchers can benefit from this, as toxins with potent effects on the body can inspire new drugs.More information on the thesis END:VEVENT END:VCALENDAR