Blood vascular and lymphatic endothelial cells in lymphoid tissues possess a remarkable array of functions. Not only are they vital to nutrient transport but they are also indispensable in immune surveillance and blood coagulation. The adaptations taken on by endothelial cells to execute their various functions are reflected in their heterogeneity. In this thesis we have explored various mechanisms that contribute to endothelial specializations. We address endothelial identity to show the importance of Notch signaling in adult capillary blood endothelial cells and COUP-TFII in adult endothelial mucosal vascular addressin expression. We identify a capillary resident precursor population in the adult peripheral lymph node blood vasculature and show the vital progenitor function of this endothelial subset in the lymphoid vascular remodeling process. Further, production of the coagulation factor VIII by the high endothelial venules and the lymphatic endothelial cells sheds light on the clotting function of the lymphoid endothelium. These findings highlight lymphoid endothelial cells are more heterogenous with a wider array of functions than previously thought. This thesis significantly adds to the existing knowledge of lymphoid endothelial biology, making potential endothelial cell targeted therapies more available for use, from bleeding disorders to inflammatory diseases.
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